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isotype controls  (Proteintech)


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    Structured Review

    Proteintech isotype controls
    Isotype Controls, supplied by Proteintech, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complement+factor+h/Complement+factor+H+Antibody/pm41781748-285-24-20
    Average 88 stars, based on 4 article reviews
    isotype controls - by Bioz Stars, 2026-11
    88/100 stars

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    Related Articles

    Staining:

    Article Title: Intracellular complement Factor H promotes tumor progression through modulation of cell cycle and actin cytoskeleton.
    Article Snippet: .. Cells were stained with 1:50 α-p53 mAb (SantaCruz sc126) or anti-FH OX24 (Mouse, expressed in house) or anti-FH rabbit polyclonal (Proteintech, ref 12748-1-AP) or isotype controls (Dako FLEX negative control mouse and rabbit cocktail, catalog no IR750 and IR600 respectively) overnight at 4°C, and then with Phalloidin-IFluor 488 reagent (Abcam ab176753) for 90 minutes in the dark. .. were used 70,71 at different concentrations ranging from 500 nM to 15.62 nM or 250 μM to 7.8 nM diluted in either normal or low ionic strength buffers.Data were analyzed using BIAevaluation software (version 4.1; Biacore, GE Healthcare) and the binding to the empty chip sensor surface was subtracted to estimate the kinetic binding constants.

    Negative Control:

    Article Title: Intracellular complement Factor H promotes tumor progression through modulation of cell cycle and actin cytoskeleton.
    Article Snippet: .. Cells were stained with 1:50 α-p53 mAb (SantaCruz sc126) or anti-FH OX24 (Mouse, expressed in house) or anti-FH rabbit polyclonal (Proteintech, ref 12748-1-AP) or isotype controls (Dako FLEX negative control mouse and rabbit cocktail, catalog no IR750 and IR600 respectively) overnight at 4°C, and then with Phalloidin-IFluor 488 reagent (Abcam ab176753) for 90 minutes in the dark. .. were used 70,71 at different concentrations ranging from 500 nM to 15.62 nM or 250 μM to 7.8 nM diluted in either normal or low ionic strength buffers.Data were analyzed using BIAevaluation software (version 4.1; Biacore, GE Healthcare) and the binding to the empty chip sensor surface was subtracted to estimate the kinetic binding constants.

    Incubation:

    Article Title: Mutations in UMOD Contribute to the Pathogenesis of ADTKD-UMOD by Influencing the Function of Complement Factor H.
    Article Snippet: .. The sections were blocked with 3% bovine serum albumin (BSA) for 1 h, followed by incubation with primary antibodies, including cFB (Proteintech, China), C3 (Dako, Denmark), C1q (Dako, Denmark) and IgG (Southern Biotech, USA), at 37°C for 30 min. ..

    Article Title: Mutations in UMOD Contribute to the Pathogenesis of ADTKD ‐ UMOD by Influencing the Function of Complement Factor H
    Article Snippet: .. The sections were blocked with 3% bovine serum albumin (BSA) for 1 h, followed by incubation with primary antibodies, including cFB (Proteintech, China), C3 (Dako, Denmark), C1q (Dako, Denmark) and IgG (Southern Biotech, USA), at 37°C for 30 min. ..

    Article Title: Innovative Dual mRNA-Lipid Nanoparticle Therapy Targeting CRHBP and CFHR3 for Enhanced Treatment of Hepatocellular Carcinoma
    Article Snippet: .. Primary antibodies against CFHR3 (1:100, 16583-1-AP, Proteintech), CRHBP (1:100, A6568, ABclonal), and Ki67 (1:5000, HA721115, Huabio) were incubated overnight at 4°C. ..

    Article Title: A study on the establishment of a traumatic brain injury model of tree shrews and the observation of neuroinflammatory responses
    Article Snippet: Total protein was separated by 10 % SDS-PAGE and transferred onto a polyvinylidene fluoride (PVDF) membrane (Millipore, Billerica, USA). .. Membranes were blocked with 5 % BSA at room temperature for 2 h and incubated overnight at 4°C with primary antibodies: Serping1 (66882–1-Ig, Proteintech, 1:1000), Ptx3 (13797–1-AP, Proteintech, 1:1000), and rabbit anti-β-actin (20536–1-AP, Proteintech, 1:5000). ..

    Reverse Transcription:

    Article Title: Qiju Dihuang Pill modulates complement system in age-related dry eye disease: Tripartite validation through clinical-omics-animal integration.
    Article Snippet: Ethnopharmacological relevance: Qiju Dihuang Pill (QJDHW) is a traditional Chinese medicine (TCM) formulation used for treating ocular diseases, and clinical evidence has demonstrated its efficacy in managing dry eye disease (DED).. Age-related DED is characterized by tear film instability and ocular surface dysbiosis.. Current therapeutic agents face challenges in achieving sustained efficacy.

    Marker:

    Article Title: Qiju Dihuang Pill modulates complement system in age-related dry eye disease: Tripartite validation through clinical-omics-animal integration.
    Article Snippet: Ethnopharmacological relevance: Qiju Dihuang Pill (QJDHW) is a traditional Chinese medicine (TCM) formulation used for treating ocular diseases, and clinical evidence has demonstrated its efficacy in managing dry eye disease (DED).. Age-related DED is characterized by tear film instability and ocular surface dysbiosis.. Current therapeutic agents face challenges in achieving sustained efficacy.

    Stripping Membranes:

    Article Title: Qiju Dihuang Pill modulates complement system in age-related dry eye disease: Tripartite validation through clinical-omics-animal integration.
    Article Snippet: Ethnopharmacological relevance: Qiju Dihuang Pill (QJDHW) is a traditional Chinese medicine (TCM) formulation used for treating ocular diseases, and clinical evidence has demonstrated its efficacy in managing dry eye disease (DED).. Age-related DED is characterized by tear film instability and ocular surface dysbiosis.. Current therapeutic agents face challenges in achieving sustained efficacy.



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    Image Search Results


    FHR5 1-9 FH 1-5 binds to C3, destabilizes C3 convertase and acts as a cofactor for factor I. Binding profiles of increasing amounts of FHR5 1-9 FH 1-5 to surface bound C3, C3b, iC3b, C3d and BSA (negative control), detected with both anti-CFHR5 (a) and anti-FH (b) antibodies. Data shown are mean values of triplicate measurements; error bars denote the standard deviation. C3a generated by convertase formation on surface-bound C3b: ELISA plates were coated with purified C3b. Surface C3 convertase was formed by addition of purified factor B (FB), factor D (FD), and properdin (P). Then increasing amounts of either FH (c) or the FHR5 1-9 FH 1-5 (d) were added followed by addition of C3 to enable C3a generation. To investigate factor I (FI) co-factor activity, FI along with increasing amounts of either FH (e) or the FHR5 1-9 FH 1-5 (f) were added prior to the convertase formation step. NC—negative control, no FB, FD, or P added. PC—positive control, no FH, or FHR5 1-9 FH 1-5 added. P values were determined by one-way ANOVA with Dunnett’s multiple comparisons test; **** P < 0.0001. Fluid phase co-factor assay products were visualized by western blotting (g). Cofactor activity was indicated by the cleavage of C3b as determined by the presence of C3 α-chain fragments (arrow). Negative controls: C3, C3b, C3b with FH, C3b with FI, and FHR5 1-9 FH 1-5 alone. Positive control: C3b incubated with FH and FI.

    Journal: Clinical and Experimental Immunology

    Article Title: A novel fusion protein reduces kidney complement in experimental C3 glomerulopathy

    doi: 10.1093/cei/uxag015

    Figure Lengend Snippet: FHR5 1-9 FH 1-5 binds to C3, destabilizes C3 convertase and acts as a cofactor for factor I. Binding profiles of increasing amounts of FHR5 1-9 FH 1-5 to surface bound C3, C3b, iC3b, C3d and BSA (negative control), detected with both anti-CFHR5 (a) and anti-FH (b) antibodies. Data shown are mean values of triplicate measurements; error bars denote the standard deviation. C3a generated by convertase formation on surface-bound C3b: ELISA plates were coated with purified C3b. Surface C3 convertase was formed by addition of purified factor B (FB), factor D (FD), and properdin (P). Then increasing amounts of either FH (c) or the FHR5 1-9 FH 1-5 (d) were added followed by addition of C3 to enable C3a generation. To investigate factor I (FI) co-factor activity, FI along with increasing amounts of either FH (e) or the FHR5 1-9 FH 1-5 (f) were added prior to the convertase formation step. NC—negative control, no FB, FD, or P added. PC—positive control, no FH, or FHR5 1-9 FH 1-5 added. P values were determined by one-way ANOVA with Dunnett’s multiple comparisons test; **** P < 0.0001. Fluid phase co-factor assay products were visualized by western blotting (g). Cofactor activity was indicated by the cleavage of C3b as determined by the presence of C3 α-chain fragments (arrow). Negative controls: C3, C3b, C3b with FH, C3b with FI, and FHR5 1-9 FH 1-5 alone. Positive control: C3b incubated with FH and FI.

    Article Snippet: After incubation and washing, bound FHR5 1-9 FH 1-5 was detected using goat polyclonal anti-CFHR5 antibody (R&D systems), followed by mouse anti-goat/sheep IgG-HRP (Sigma) and finally TMB substrate (BD).

    Techniques: Binding Assay, Negative Control, Standard Deviation, Generated, Enzyme-linked Immunosorbent Assay, Purification, Activity Assay, Positive Control, Western Blot, Incubation